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genechip microarray analysis suite  (Thermo Fisher)


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    Thermo Fisher genechip microarray analysis suite
    Genechip Microarray Analysis Suite, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genechip microarray analysis suite/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    genechip microarray analysis suite - by Bioz Stars, 2026-04
    90/100 stars

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    Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the microarray results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Alteration of Gene Expression in Pathological Keratinization of the Ocular Surface

    doi: 10.1167/iovs.65.6.37

    Figure Lengend Snippet: Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the microarray results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.

    Article Snippet: Microarray experiments were then performed according to the protocols provided by Thermo Fisher Scientific, and scanned microarray images were obtained using the GeneChip Scanner 3000 7G (Thermo Fisher Scientific) microarray analysis system.

    Techniques: Quantitative RT-PCR, Binding Assay, Expressing, Microarray